Part:BBa_K608351

(correct) temperature sensitive promoter

This is based on part (BBa_K098995)

Why did we make this part?: Because sequencing of (BBa_K098995) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor (BBa_K098997) CDS.

Usage and Biology

This composite promoter consists of a constitutive promoter (BBa_J23114), the temperature sensitive λ cI repressor protein (BBa_K098997) which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised to 42°C, and a cI regulated promoter based on the λ pR promoter (BBa_R0051).

The temperature sensitive promoter functioning at 28-30°C

The temperature sensitive promoter functioning at 42°C

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters: Austin_UTexas

BBa_K608351 parameters

Burden Imposed by this Part:

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.